Composite

Part:BBa_K1792002:Design

Designed by: Brian Dempsey   Group: iGEM15_Lethbridge_HS   (2015-09-17)


alpha-Dextranase, secreted


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1782
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 478


Design Notes

This is a very large gene (1,818 nucleotides, native sequence). The Nterminal 14 amino acids are not included due to disorder. An bacterial Nterm export tag is included in the sequence. This protein has a Cterminal 6XHis tag.


Source

This sequence is from the UniprotKB database entry: R4UBH6 (R4UBH6_9PEZI) and is a genomic sequence. alpha-Dextranase from Chaetomium gracile (Fungi).

References